Organic synthesis: development new methods for stereoselective coupling of carbohydrate units.

Carbohydrate chemistry: target-orientated oligosaccharides synthesis and synthesis of glycolipids.

Synthesis and design of glycoconjugates for study of biological properties.

Research interests:

The research in Mong lab aims on the development of practically simple synthetic methods for the synthesis of biologically potent but commercially non-available monosaccharaides, oligosaccharides, neoglycoconjugates, cell wall components of pathogenic bacteria, and natural glycosylated products via a combination of organocatalytic synthesis, solvent participation in glycosylation, nucleophile-modulated glycosylation approach. Major research effort devotes to four topics:

1. Discovery of new methods and chemistry for synthesis of natural glycoconjugates, inner core oligosaccharides of lipopolysaccharides, lipid A with various of fatty acid chains, O-antigens of LPS for immunological studies.
2. Preparation of anionic oligosaccharides including PI-88, hyaluronic acid, sulfated LacNAc oligomers to investigate the binding properties for protein receptors in human cell-lines.
3. Preparation of glycodendrimers and glyconanoparticles for development of bacteria sensing devices and study the carbohydrate-protein interactions.
4. Chemical synthesis of natural phenylethanoid glucosides and their structural analogues for study of anti-proliferation properties on human cancer cell-lines.

Collaborators:

1. Prof. Chun-Hung Lin, Institute of Biological Chemistry, Academia Sinica
2. Prof. Yu-Chie Chen, Department of Applied Chemistry, National Chiao Tung University

Equipments:

1. HPLC and Gel Permeation chromatography
2. Precision light-scattering analyzer
3. Low temperature reactors -80^oC to RT
4. FPLC purification system
5. Solvent purification system

National Science and Technology Council Research Projects:

8/2023-7/2025

Design and Synthesis of Toll-like Receptor 4 Agonists and Antagonists with a 1,1-linked Trehalosamine Scaffold

8/2021-7/2023

Exploration on New Reverse Orthogonal Glycosylation Method with Heptosyl Phosphate Acceptors and its Application for One-pot Synthesis of Inner Core Oligosaccharide of Lipopolysaccharide for Yersinia pestis

8/2019-7/2021

Synthesis of new nonsymmetrical trehalose and skipped methyl  deoxypolypropronate fatty acid components of Mycobacterium tuberculosis related sulfo-glycolipids

8/2017-7/2019

Total Synthesis of “Trojan Horse” Antibiotics, Salmycins Based on New Homologation Strategy for Sugar Substrates

8/2016-7/2019

Structure-activity relationship of cholesteryl glucoside derivatives to study CagA translocation and lipid raft formation

8/2016-7/2017

Total Synthesis of Landomycin Antibiotics on the base of CH Activation and Remote Control Glycosylation Methods

8/2013-7/2016

Exploration of alpha-Glycosylation method for inactive 2-Azido-2-Deoxy-thioglycoside donors

8/2011-7/2014

Synthesis of structurally defined functional carbohydrates for probing foodborne pathogens and toxins

8/2010-7/2012

Resolution of aglycon transfer of thioglycosides in orthogonal glycosylations and development of multiple component one-pot glycosylation (MuCOP) strategy

8/2008-7/2010

Synthesis of Neoglycodendritic proteins as chemical probes for protein receptor

8/2007-7/2008

New synthetic strategy towards cancer related Lewis antigen hapten

11/2005-7/2007

Design and synthesis of polymeric glycoprotein hybrid